Materials for auditory self-work.
4.1. List of study practical tasks necessary to perform at the practical class.
Materials and methods: scarificator, pipettes, blood dilutor, Panchenkov’s device, test tubes, centrifuge, blood.
Investigation object: rat.
Task 1. To get acquainted with blood taking technology for analysis performance.
The investigator must wipe investigated person left fourth finger with cotton wool washed in alcohol. To prick the cured finger with sterile scarificator. To dry first blood drop with cotton wool. To put the end of horizontally located pipette in the second blood drop and to fetch very carefully (without vesicles) blood till corresponding mark. To gain this goal it’s necessary to immerse the pipette in complete blood drop. It’s necessary to dry pipette end with cotton wool and to perform manipulations with blood according to investigation character.
Task 2. To determine erythrocytes osmotic resistance.
To put 20 test tubes in support and to number them. To pour hypotonic solutions in the dosage of 1 ml in corresponding test tubes (concentrations from 0,60 to 0,15 per cent with the difference in 0,05 per cent in every test tube). To add investigated blood in every test tube. To mix carefully the content and to stay them at room temperature for 15 minutes. To centrifugate after this at 1500 rotations per minute in course of 5 minutes. To determine the limits of erythrocytes maximal and minimal resistance.
Erythrocytic resistance - is red blood cells feature to resist injured actions (osmotic, chemical, mechanical and so on). But osmotic resistance to sodium chloride hypotonic solutions is the most-spread resistance index determined under clinical conditions (it is rather easily to be performed and to evaluated comparatively to other resistance types).
Under norm minimal resistance (hemolysis beginning) – at sodium chloride content 0,42-0,48 per cent; maximal resistance (complete hemolysis) – at 0,30-0,34 per cent of it. In a fresh blood- 0,20-0,40 per cent of NaCl; in incubated one (in course of 24 hours) – 0,20-0,65 per cent of NaCl.
It is decreased at:
· congenital microspherocytic hemolytical jaundice;
· new-borns haemolytical disease;
· toxicoses;
· acute infections;
· leukemias;
· lymphogranulematoses;
· hepatic cirrhoses;
· ABO- and Rh- blood incompatibility.
It is increased at:
· drepanocytic anaemias;
· mechanical jaundices.
But this index is the most significant at anemias differentiated diagnosis.
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Task 3. Velocity sedimentation rate (VSR) determining.
To wash capillary pipette of Panchenkov with 5% solution of citrate sodium. To fetch this solution till the mark 75/25 mm3 and to blow it to the clock glass.
To prepare the finger, to prick it and to fetch blood till the mark 100 mm3. To blow blood to the clock glass and to mix it with citrate sodium in correlation of 1:4. To fill the pipette with this citric blood exactly till the mark “K” and to put it into support vertically for 1 hour. In 1 hour to determine the highland in mm of plasma column above formed elements.
2. Literature recommended:
1. Lecture course.
2. Mistchenko V.P., Tkachenko E.V. Methodical instructions for dental students (short lecture course).-Poltava, 2005.-P.38-39.
3. Mistchenko V.P., Tkachenko E.V. Methodical instructions for medical students (short lecture course).-Poltava, 2005.-P. 60-62.
4. Mistchenko V.P., Tkachenko E.V. Blood system Physiology //Methodical recommendations to practical classes for students of medical and dental departments.-Poltava, 2005.-20 p.
5. Kapit W., Macey R.I., Meisami E. The Physiology Colouring Book: Harpers Collins Publishers, 1987.-P. 135.
6. Guyton – Ganong – Chatterjee. Concise Physiology /Ed. By Dr Raja Shahzad Gull: M.B.B.S., F.C.P.S., King Edward Medical College.-Lahore, 1998 (1st Edition).-P.170-171, 203-204.
7. Stuart Ira Fox. Human Physiology.-8th Ed.-McGrawHill, 2004.-P.367-368, 377-378.
8. Seeley R.R., Stephens T.D., Tate P. Essentials of Anatomy and Physiology.-The 3rd Ed.-McGraw Hill, 1999.-P.286-288.
2. Materials for self-control:
Control questions:
1. Blood system.
2. Blood content, its amount.
3. Blood functions.
4. Blood constants and their significance in clinical practice.
5. Blood main components, hematocrit.
6. Blood buffer systems, acidosis, alkalosis.
7. Erythrocytic osmotic resistance.
8. Velocity sedimentation rate, factors, influencing on it, diagnostic value.
9. Osmotic pressure.
10. Oncotic pressure.
11. Blood viscosity.
12. Blood temperature, blood color, factors they depend on.
LESSON 32
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