Isolating pure bacterial cultures
THE MINISTRY OF EDUCATION ANDSCIENCEOF RF
Federal State Education Institution of Higher Professional Education
“Penza State University”
Department “History OF Medicine”
ROBERT KOCH (1843-1910)-Formulated Koch’s postulates
NAME: Rutuja Kude
GROUP NO:19LL3(a)
DOCTOR’S NAME: GAVRILOVA TATYANA
Contents
Biography, Life.
Contribution to medicine
.
BIOGRAPHY OF ROBERT KOCH: -
Koch was born in Clausthal, Germany, on 11 December 1843, to Hermann Koch (1814–1877) and Mathilde Julie Henriette (née Biewend; 1818–1871). Koch excelled academically from an early age. Before entering school in 1848, he had taught himself how to read and write. He graduated from high school in 1862, having excelled in science and math. At the age of 19, Koch entered the University of Göttingen, studying natural science. However, after three semesters, Koch decided to change his area of study to medicine, as he aspired to be a physician. During his fifth semester of medical school, Jacob Henle, an anatomist who had published a theory of contagion in 1840, asked him to participate in his research project on uterine nerve structure. In his sixth semester, Koch began to research at the Physiological Institute, where he studied the secretion of succinic acid, which is a signaling molecule that is also involved in the metabolism of the mitochondria. This would eventually form the basis of his dissertation. In January 1866, Koch graduated from medical school, earning honors of the highest distinction.
Life
Heinrich Hermann Robert Koch 11 December 1843 – 27 May 1910) was a German physician and microbiologist. As one of the main founders of modern bacteriology, he identified the specific causative agents of tuberculosis, cholera, and anthrax and also gave experimental support for the concept of infectious disease, which included experiments on humans and animals. Koch created and improved laboratory technologies and techniques in the field of microbiology, and made key discoveries in public health. His research led to the creation of Koch's postulates, a series of four generalized principles linking specific microorganisms to specific diseases that proved influential on subsequent epidemiological principles such as the Bradford Hill criteria. For his research on tuberculosis, Koch received the Nobel Prize in Physiology or Medicine in 1905. The Robert Koch Institute is named in his honour.
|
|
|
In about 1501, he was called to court as tutor of the young Arthur, Prince of Wales. On the accession of Henry VIII in 1509, he was appointed the king's physician, an office at that time of considerable influence and importance, and practised medicine in London, having among his patients most of the great statesmen and prelates of the time, including Cardinal Wolsey, Archbishop William Wareham and Bishop Fox.
After some years of professional activity, Linacre devoted himself to the study of theology and the duties of the priesthood. Around 1509, he received priest's orders as the rector of Merstham, Kent. Numerous ecclesiastical positions followed, finalising with him obtaining the rector ship of Wigan in 1520, which he held until his death in 1524. His clerical benefices, included the Precentor ship of York Minster. His ordination was connected with his retirement from active life. Literary labours, and the cares of the foundation which owed its existence chiefly to him, the Royal College of Physicians, occupied Linacre's remaining years.
Contribution to medicine and his work
Several years after his graduation in 1866, he worked as a surgeon in the, and following his service, worked as a physician in Wollstein in prussian posen (now wolystyn, Poland). From 1880 to 1885, Koch held a position as government advisor with the Imperial Department of Health. Koch began conducting research on microorganisms in a laboratory connected to his patient examination room. Koch's early research in this laboratory yielded one of his major contributions to the field of microbiology, as he developed the technique of growing bacteria. Furthermore, he managed to isolate and grow selected pathogens in pure laboratory culture.
|
|
|
From 1885 to 1890, he served as an administrator and professor at berlin university.
In 1891, Koch relinquished his Professorship and became a director of the medical institute which consisted of a clinical division and beds for the division of clinical research. For this he accepted harsh conditions. The Prussian Ministry of Health insisted after the 1890 scandal with tuberculin, which Koch had discovered and intended as a remedy for tuberculosis, that any of Koch's inventions would unconditionally belong to the government and he would not be compensated. Koch lost the right to apply for patent protection.
Research
Isolating pure bacterial cultures
In an attempt to grow bacteria, Koch began to use solid nutrients such as potato slices. Through these initial experiments, Koch observed individual colonies of identical, pure cells. He found that potato slices were not suitable media for all organisms, and later began to use nutrient solutions with gelatin. However, he soon realized that gelatin, like potato slices, was not the optimal medium for bacterial growth, as it did not remain solid at 37 °C, the ideal temperature for growth of most human pathogens. As suggested to him by Walther and Fanny Hesse, Koch began to utilize agar to grow and isolate pure cultures, because this polysaccharide remains solid at 37 °C, is not degraded by most bacteria, and results in a transparent medium.
Koch's four postulates
Main article: Koch's postulates
During his time as government advisor, Koch published a report, in which he stated the importance of pure cultures in isolating disease-causing organisms and explained the necessary steps to obtain these cultures, methods which are summarized in Koch's four postulates. Koch's discovery of the causative agent of anthrax led to the formation of a generic set of postulates which can be used in the determination of the cause of most infectious diseases.These postulates, which not only outlined a method for linking cause and effect of an infectious disease but also established the significance of laboratory culture of infectious agents, are listed here:
|
|
|
The organism must always be present, in every case of the disease.
1. The organism must be isolated from a host containing the disease and grown in pure culture.
2. Samples of the organism taken from pure culture must cause the same disease when inoculated into a healthy, susceptible animal in the laboratory.
3. The organism must be isolated from the inoculated animal and must be identified as the same original organism first isolated from the originally diseased host.
Anthrax
Robert Koch is widely known for his work with anthrax, discovering the causative agent of the fatal disease to be Bacillus anthracis. He discovered the formation of spores in anthrax bacteria, which could remain dormant under specific conditions. However, under optimal conditions, the spores were activated and caused disease. To determine this causative agent, he dry-fixed bacterial cultures onto glass slides, used dyes to stain the cultures, and observed them through a microscope. His work with anthrax is notable in that he was the first to link a specific microorganism with a specific disease, rejecting the idea of spontaneous generation and supporting the germ theory of disease.
During his time as the government advisor with the Imperial Department of Health in Berlin in the 1880s, Robert Koch became interested in tuberculosis research. At the time, it was widely believed that tuberculosis was an inherited disease. However, Koch was convinced that the disease was caused by a bacterium and was infectious, and tested his four postulates using guinea pigs. Through these experiments, he found that his experiments with tuberculosis satisfied all four of his postulates. In 1882, he published his findings on tuberculosis, in which he reported the causative agent of the disease to be the slow-growing mycobacterium tuberculosis. Later, Koch's attempt at developing a drug to treat tuberculosis, tuberculin, led to a scandalous failure: he did not divulge the exact composition, and the claimed treatment success did not materialize; the substance is today used for tuberculosis diagnosis.
|
|
|
Koch and his relationship to paul ehlrich, who developed a mechanism to diagnose TB, were portrayed in the 1940 movie.
Cholera
Koch next turned his attention to cholera, and began to conduct research in egypt in the hopes of isolating the causative agent of the disease. However, he was not able to complete the task before the epidemic in Egypt ended, and, after a short trip to Persia, traveled to India to continue with the study. In 1884 in Bombay state of India (the present day State of Maharashtra,India), Koch resided and researched at Grant Medical College, (or by some accounts in Kolkata, formerly Calcutta in undivided British India) where he was able to determine the causative agent of cholera, isolating Vibrio cholera. The bacterium had originally been isolated in 1854 by Italian anatomist Filipo Pacinni, but its exact nature and his results were not widely known.
Дата добавления: 2021-11-30; просмотров: 31; Мы поможем в написании вашей работы! |
Мы поможем в написании ваших работ!